Complete Guide to Enzyme Linked Immunosorbent Assay (ELISAs)

Master Immuno assays for diagnostics, protocols, quality controls, troubleshooting and statistical data interpretations

Master Immuno assays for diagnostics, protocols, quality controls, troubleshooting and statistical data interpretations

Overview

ELISAs Fundamentals, Applications and Data Analysis, Enzyme Linked Immunosorbent Assays, Design Protocols for Diagnostics and Drug Discovery, Troubleshooting and Quality Control for Assays

Molecular biology and immunology researchers, Students in biomedical and life sciences, Biopharma and diagnostic assay developers, This course is designed for: Undergraduate & graduate students in biology, biotechnology, biochemistry, immunology, and related fields looking to strengthen their laboratory skills. Research scientists and technicians seeking a structured, detailed, and practical refresher on ELISA design, execution, and data interpretation. Diagnostic lab professionals working with ELISA in clinical, pharmaceutical, or quality control environments. Biotech and pharmaceutical industry employees involved in assay development, validation, or regulatory documentation. Educators and trainers who need a complete, scientifically accurate resource to teach ELISA fundamentals and modern advancements. Anyone preparing for laboratory interviews or career transitions where ELISA expertise is essential., Clinical laboratory technicians and technologists, Professionals seeking ELISA troubleshooting expertise

Basic understanding of biology or chemistry recommended but not required

This comprehensive ELISA training course equips clinical laboratory professionals, researchers, and students with the skills to master enzyme-linked immunosorbent assay techniques for diagnostic applications.

Through four detailed modules, you will explore antibody structure and antigen-binding principles, master standardized ELISA protocols (direct, indirect, sandwich, and competitive formats), and apply best practices in protein immobilization, enzyme detection, and blocking strategies.

You will learn to implement robust quality control measures, troubleshoot common assay issues, and apply statistical analysis for accurate interpretation of diagnostic results. Each lesson is designed to enhance both theoretical understanding and hands-on competence, ensuring you can confidently run ELISAs in clinical, research, and industrial settings.

Whether you work in molecular biology, immunology, biopharma, or clinical diagnostics, this course will help you improve assay reliability, minimize variability, and produce reproducible, regulatory-compliant results. You will also gain insights into advanced applications such as ELISpot, bead-based assays, digital ELISA, and aptamer-based detection—expanding your ability to choose the right format for your research or diagnostic needs.

By the end of this course, you will be able to confidently execute ELISA protocols, analyze results using statistical models, and apply troubleshooting strategies to ensure assay success—making you a valuable contributor to any diagnostic or research team, and enhancing your career prospects in biomedical sciences.

Dr. Amogh Gopinathan Nair

Dr. Amogh Gopinathan Nair, Ph.D


Immuno-Oncology & Stem Cell Scientist | Antibody Engineering & AI-Driven Drug Discovery | Regulatory Affairs & Translational Research Expert | 15+ Years in Academia & Industry | Merck-MITACS and Lonza-Trained


Hello everyone, I am Dr. Amogh Nair, a scientist-educator with over 15 years of experience at the intersection of cutting-edge biology and real-world drug development—from discovery to IND-enabling studies, clinical trial design, and early market access. Trained in both academic rigor and industry precision, I specialize in therapeutic antibody engineering, immuno-oncology, stem cell technologies, AI-enhanced drug discovery, and regulatory strategy.

My professional journey has taken me from GMP-driven cell line development at Lonza to CRISPR-edited iPSC models at the University of Sheffield, and from multi-specific antibody development in clinical-stage oncology programs to regulatory interactions with global health authorities.


Areas of Expertise:


Antibody Engineering (hybridoma, bi-specifics, ADCs, CAR-T)

Stem Cells & Organoids (hESCs, iPSCs, CRISPR/TALEN, disease modeling)

Immuno-Oncology (tumor biology, MOA studies, biomarker integration)

AI in Drug Discovery (epitope prediction, HT screening, lead optimization)

Regulatory Affairs (preclinical strategy, IND packages, trial design, market access)


As Your Instructor, I Offer:


Clarity & Context – I break down complex topics into actionable insights for biotech, clinical, and regulatory learners.

Real-World Relevance – Every concept connects to current therapeutics, real drug programs, and actual regulatory decisions.

Career-Ready Knowledge – Whether you are transitioning from academia or upskilling in biotech, I teach with the mindset of industry readiness.


Featured Course Topics:


Antibody Engineering for Oncology and Cardio-metabolic diseases (GLP-1, GIP, GCGR) – structure, function, platforms, clinical relevance

CAR-T & Biologics – core concepts, applications, safety, and scalability

AI & Drug Discovery – predictive tools, pipeline acceleration, integration with wet-lab research

Stem Cell Biology in MedicineiPSC technologies, Organoids, “clinical trials in a dish” and AI integration.

Regulatory Pathwayshow therapeutics move from bench to bedside


Outside the lab, I am a dedicated trail runner and culinary explorer—both of which teach the same lesson I bring into the classroom: exploration, precision, patience, and purpose.


If you are seeking an instructor who brings deep scientific knowledge, cross-functional experience, and a passion for clear, impactful teaching, you are in the right place.

Go ahead, learn the skills and explore.

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